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CP Canadian PeptidesReference Standards
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Bench work

Reconstitution basics: math, diluents, and aliquotting

The arithmetic behind preparing a working stock from a lyophilized reference vial, and why aliquotting is worth the extra ten minutes.

Pick a target stock

Choose a working-stock concentration that suits your assay's typical draw volume. A common choice is one that lets a 50–200 µL draw deliver the mass you need per characterisation run.

Calculate the diluent volume

Diluent volume in mL = vial mass in mg ÷ target concentration in mg/mL. Our reconstitution calculator does this for you and also reports the equivalent volume on a U-100 syringe scale, for compatibility with common lab tooling.

Choosing a diluent

  • Bacteriostatic water is a common default for water-soluble peptides.
  • For poorly soluble compounds, a small percentage of DMSO followed by aqueous dilution is common; check the molecule's published solubility before choosing.
  • Always vortex gently or invert; aggressive shaking can denature peptide structure.

Aliquot the prepared stock

Split the reconstituted stock into single-use aliquots in low-bind tubes. Each freeze-thaw on a prepared stock degrades it; aliquotting eliminates repeated freeze-thaw entirely.

In-vitro research only

This article is reference material for qualified research professionals. It is not medical, clinical, or diagnostic guidance. Reference standards are sold for in-vitro characterisation only.

Continue reading
Handling and storage of lyophilized reference standards
How to keep a sealed reference vial stable from receiving dock to bench, and what changes once you reconstitute it.
How to read a Certificate of Analysis
A line-by-line walkthrough of the COA we ship with every lot — what each field means and which numbers actually matter for your bench work.
Lab safety etiquette around reference standards
PPE, fume-hood protocol, waste handling, and incident-response habits that keep a reference-standard bench safe and reproducible.
Glossary: terms you will see on a COA and in catalog copy
Quick definitions for COA, lyophilizate, HPLC, MS, BAC water, and other terms that show up across the catalog.