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Lab handling

Handling and storage of lyophilized reference standards

How to keep a sealed reference vial stable from receiving dock to bench, and what changes once you reconstitute it.

Receiving a sealed vial

A lyophilized reference standard arrives sealed under inert atmosphere in a butyl-stoppered, crimp-capped vial. The vial is shipped in cold-pack; once it reaches your bench, allow the vial to equilibrate to room temperature inside its outer packaging before inspecting the seal.

Inspecting the cake while the vial is still cold can pull moisture in through any micro-defect in the seal. Patience here protects the lot integrity.

Long-term storage

  • Sealed vial: store at -20 °C, protected from light, in a low-humidity freezer.
  • Short-term (up to 14 days): refrigerate at 2–8 °C in the original sealed vial.
  • Avoid repeated freeze-thaw of a sealed lyophilizate; pull only when ready to characterise.

After reconstitution

Once reconstituted, store the prepared stock per your in-vitro protocol's stability window for the molecule. Aliquot to minimise repeated freeze-thaws of the prepared stock.

Document the reconstitution date and the diluent so downstream characterisation runs are traceable.

In-vitro research only

This article is reference material for qualified research professionals. It is not medical, clinical, or diagnostic guidance. Reference standards are sold for in-vitro characterisation only.

Continue reading
How to read a Certificate of Analysis
A line-by-line walkthrough of the COA we ship with every lot — what each field means and which numbers actually matter for your bench work.
Reconstitution basics: math, diluents, and aliquotting
The arithmetic behind preparing a working stock from a lyophilized reference vial, and why aliquotting is worth the extra ten minutes.
Lab safety etiquette around reference standards
PPE, fume-hood protocol, waste handling, and incident-response habits that keep a reference-standard bench safe and reproducible.
Glossary: terms you will see on a COA and in catalog copy
Quick definitions for COA, lyophilizate, HPLC, MS, BAC water, and other terms that show up across the catalog.